Amino acid sequence alignment of representative of pilosulins and pilosulin-like from different ant species. The sequence of bicarinalin/pilosulin-like (Tb34317) from T. bicarinatum was aligned with sequences of the pilosulin family from Myrmecia banksi [GenBank: Q68Y23.1 and BAF95069] and Myrmecia pilosula [GenBank: AAB50883]. JZ168521–JZ168537 refer to GenBank accession numbers of transcripts from T. bicarinatum contig recovered from our previous study . Amino acids that are identical in all or the majority of the sequences are highlighted with different shades of blue that represent their degree of conservation. The active peptide sequence of bicarinalin identified in a previous study  is indicated by a red box. Putative signal peptide is underlined.