Figure 4

Analysis of adenine methylation on the secondary endosymbiont’s helicase gene. Schematic representation of the A6 fragment (Regiella insecticola helicase gene, accession number WP_006705384.1). Orange circles represent the positions of the examined adenine methylation sites (A6M1–A6M3); arrows indicate primer positions and orientations (a). PCR amplification of digested gDNA from individual A. pisum aphids (orange and green variants) using A6F3 and A6R2 primers (b-c), A6F1 and A6R1 primers (d-e), and A6F2 and A6R2 primers (f-g). Comparison of the cDNA expression levels in orange and green variants of the A6 fragment (h), and R. insecticola 16S (i).