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Table 1 Summary of adenine methylation on the secondary endosymbiont’s transposase gene

From: Adenine methylation may contribute to endosymbiont selection in a clonal aphid population

Primers

 

A1F & A1R1

 

A1F2 & A1R1

 

Phenotype

*No. of aphids

DpnI

DpnII

DpnI + DpnII

DpnI

DpnII

DpnI + DpnII

22°C( Orange )

21

(21)+++

(21)+++

(21)+++

(17)+++ (4)++

(15) + (6)-

(5) + (16) -

8°C ( Green )

21

(21)+++

(21)+++

(21)+++

(3)++ (5) + (13) -

(13)+++ (6)++ (2)+

(1)++ (3) + (17) -

  1. *Number of individual Acyrthosiphon pisum aphids tested by PCR amplification. The amplicon intensity was categorized into three levels: +++, high; ++, medium; +low; -, no amplification.