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Table 1 Summary of adenine methylation on the secondary endosymbiont’s transposase gene

From: Adenine methylation may contribute to endosymbiont selection in a clonal aphid population

Primers   A1F & A1R1   A1F2 & A1R1  
Phenotype *No. of aphids DpnI DpnII DpnI + DpnII DpnI DpnII DpnI + DpnII
22°C( Orange ) 21 (21)+++ (21)+++ (21)+++ (17)+++ (4)++ (15) + (6)- (5) + (16) -
8°C ( Green ) 21 (21)+++ (21)+++ (21)+++ (3)++ (5) + (13) - (13)+++ (6)++ (2)+ (1)++ (3) + (17) -
  1. *Number of individual Acyrthosiphon pisum aphids tested by PCR amplification. The amplicon intensity was categorized into three levels: +++, high; ++, medium; +low; -, no amplification.