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Figure 9 | BMC Genomics

Figure 9

From: An improved ChIP-seq peak detection system for simultaneously identifying post-translational modified transcription factors by combinatorial fusion, using SUMOylation as an example

Figure 9

Diagram of the SUMO-1 ChIP-seq analysis workflow. Scheme used for the modified high-resolution ChIP-seq method and its validation. The literature was used to verify 17 of the top 19 SUMO-1-TF candidates. The SUMO-1-TF candidates were predicted by the following steps: (1) filtering poor and repeat reads out, and aligning reads to the human genome (hg19); (2) calling peaks using three tools MACS, T-PIC and CisGenome; (3) combining three peak sets; (4) annotating peaks using TFBS; (5) scoring and ranking SUMO-1 TF candidates; and finally (6) verifying SUMO-1 TF candidates via the literature.

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