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Table 1 Annotation result on high-coverage dataset A1

From: MetaCluster-TA: taxonomic annotation for metagenomic data based on assembly-assisted binning

Methods

Species-reference (~16.7 million reads)

Order-reference (~20.0 million reads)

 

Accurate

Higher

Incorrect

Unassigned

Accurate

Higher

Incorrect

Unassigned

MetaCluster-TA

60.9%

32.9%

4.0%

2.2%

31.8%

38.1%

22.6%

7.5%

MEGAN4 (contigs)

60.7%

12.9%

22.3%

4.1%

12.3%

13.1%

65.3%

9.3%

MEGAN4 (reads)

57.7%

14.8%

4.6%

22.8%

0.7%

0.7%

3.4%

95.2%

  1. * "Accurate" corresponds to the percentage of species-reference/order-reference reads annotated correctly, i.e., their correct species/order names of the target genomes; "Higher" corresponds to the percentage of species-reference/order-reference reads that are correctly annotated, but to taxonomy of higher levels than species/order of the target genomes (e.g. reads of E. coli-reference annotated with family name Enterobacteriaceae); "Incorrect" corresponds to the percentage of reads which are annotated incorrectly; "Unassigned" corresponds to the percentage of reads that cannot be annotated to any taxonomy.
  2. * Running time of MetaCluster-TA is about 8 hours; running time of MEGAN4 (reads) is about 4 days; running time of MEGAN4 (contigs) is about 1 day.
  3. * About 80% reads can be aligned to contigs of length > 500 bp with <5% mismatches.