Figure 1
From: TIGAR2: sensitive and accurate estimation of transcript isoform expression with longer RNA-Seq reads
The TIGAR2 pipeline. The schematic diagram shows a typical workflow of running TIGAR2 software. Alignment tools such as Bowtie2 take two input files, read data in FASTQ format and cDNA reference sequences in FASTA format. After reads are aligned to the cDNA sequence, the generated BAM file and the reference FASTA file are used in TIGAR2 for estimating transcript isoform abundances and calculating FPKMs.