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Table 1 Summary of the 10 pairs of mesophilic and thermophilic proteins used in the case study, their similarity and the environmental conditions (pH and Temperature) used to perform the test [31].

From: NeEMO: a method using residue interaction networks to improve prediction of protein stability upon mutation

 

Mesophile

Extremophile

Alignment

Protein

PDB code

Species

pH

T (°C)

PDB code

Species

pH

T (°C)

Identity

Gaps

Adenylate kinase

1AK2A

B. taurus

7

38

1ZIPA

G. stearothermophilus

7

65

90/223 (40.4%)

9

Phosphoglycerate Kinase

3PGKA

S. cerevisiae

6,6

30

1PHPA

G. stearothermophilus

7

65

210/420 (50.0%)

31

Reductase

1LVLA

P. putida

7

30

1EBDA

G. stearothermophilus

7

65

192/466 (41.2%)

19

Lactate Dehydrogenase

1LDMA

S. acanthias

7,9

11

1LDNA

G. stearothermophilus

7

65

111/335 (33.1%)

25

TATA box binding protein

1VOKA

A. thaliana

7

20

1PCZA

P. woesei

7

98

75/198 (37.9%)

22

Subtilisin

1ST3A

B. lentus

7

20

1THMA

T. vulgaris

6

60

132/282 (46.8%)

16

Carboxy Peptidase

2CTCA

B. taurus

7

38

1OBRA

T. vulgaris

6

60

93/346 (26.9%)

62

Glyceraldehyde-3-phosphate

1GADO

E. coli

7

37

1GD1O

G. stearothermophilus

7

65

194/335 (57.9%)

6

Neutral Protease

1NPCA

B. cereus

7

30

1THLA

B. thermoproteolyticus

7

80

231/318 (72.6%)

2

Phosphofructo Kinase

2PFKD

E. coli

7

37

3PFKA

G. stearothermophilus

7

65

172/320 (53.8%)

20