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Table 3 Comparison of the gene expression levels assayed by RNA sequencing and qPCR

From: RNA-sequencing analysis of Trichophyton rubrumtranscriptome in response to sublethal doses of acriflavine

ID

Gene Product Name

3 h

12 h

24 h

  

RNA-seq

qPCR

RNA-seq

qPCR

RNA-seq

qPCR

TERG_02214

Zinc carboxypeptidase

-2.59

-3.11

-3.51

-3.69

-4.20

-3.36

TERG_07861

Subtilase

-2.54

-3.91

-3.61

-5.30

-4.81

-5.31

TERG_08557

Serine carboxypeptidase

-1.54

-1.99

-0.92

-1.69

-2.14

-1.42

TERG_06573

Calcineurin regulatory subunit B

-0.78

0.41

-1.28

0.68

-2.25

-0.42

TERG_06392

Calmodulin

0.97

0.45

0.78

0.51

1.02

0.50

TERG_02198

CamK protein kinase

0.29

1.22

0.74

1.68

1.68

3.33

TERG_01281

Malate synthase

-0.67

0.03

-1.42

-1.06

-1.83

-1.16

TERG_03762

Malate dehydrogenase

1.15

1.11

1.14

2.45

2.21

2.64

TERG_01871

Acetyl-coa acetyltransferase

1.71

-0.86

1.28

-0.20

1.43

-0.16

TERG_04125

Citrate synthase

1.71

0.62

1.73

1.68

2.40

2.15

TERG_00825

Isocitrate lyase

-1.22

-0.64

-2.18

-1.50

-2.69

-2.45

TERG_01076

Aconitase

0.73

-0.95

-0.31

-0.86

-0.92

-1.31

TERG_02979

Delta(24(24(1)))-sterol reductase (Erg4)

-1.06

-0.80

-1.39

-0.86

-1.91

-0.70

TERG_03102

Sterol 24-C-methyltransferase (Erg6)

-1.31

-0.31

-0.82

-0.68

-1.54

-0.53

TERG_01252

Catalase (catalase A)

1.70

1.77

2.45

3.35

2.80

3.24

TERG_06053

Catalase (peroxisomal catalase)

6.09

1.01

7.13

4.26

6.49

4.43

TERG_08613

Multidrug resistance protein Mdr2

-1.09

-0.85

-1.70

-1.34

-2.14

-1.70

TERG_07801

ABC multidrug transporter Mdr4

-0.69

0.91

2.6

1.63

3.13

2.30