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Table 3 Drug and/or vaccine targets prioritization parameters and functional annotation of the six essential host homologous putative targets.

From: Proteome scale comparative modeling for conserved drug and vaccine targets identification in Corynebacterium pseudotuberculosis

Gene and protein codes

Official full name

Number of cavities with Drug Scorea

> 0.80

Number of cavities with Drug Scorea

> 0.60 and < 0.80

Mol. Wt

(KDa)b

Functionsc

Cellular componentd

Pathwayse

Virulencef

Cp1002_0385

Adk

Adenylate kinase

1

0

24.120

MF: Kinase, Transferase, ATP binding

BP: Nucleotide biosynthesis

EC 2.7.4.3

Cytoplasm

Purine metabolism; AMP biosynthesis via salvage pathway

Yes

Cp1002_0692

GapA

Glyceraldehyde-3-phosphate dehydrogenase A

2

1

51.918

MF: Oxidoreductase, NAD binding, NADP binding,

BP: glucose metabolic process

EC 1.2.1.13

Cytoplasm

Glycolysis/Gluconeogenesis

Yes

Cp1002_0728

GlyA

Serine hydroxymethyltransferase

2

1

46.187

MF: Methyltransferase, Transferase

BP: Amino-acid biosynthesis One-carbon metabolism

EC 2.1.2.1

Cytoplasm

Amino-acid biosynthesis; glycine biosynthesis; One-carbon metabolism; tetrahydrofolate interconversion.

Yes

Cp1002_0738

FumC

Fumaratehydratase class II

2

0

49.767

MF: Lyase

BP: Tricarboxylic acid cycle

EC 4.2.1.2

Cytoplasm

Carbohydrate metabolism; tricarboxylic acid cycle;

(S)-malate from fumarate

Yes

Cp1002_1005

Gnd

6-phosphogluconate dehydrogenase

3

5

53.669

MF: Oxidoreductase

BP: Pentose shunt

EC 1.1.1.44

Cytoplasm

Carbohydrate degradation; pentose phosphate pathway;

No

Cp1002_1042

AspA

Aspartate ammonia-lyase

2

4

52.277

MF: Lyase

EC 4.3.1.1

Cytoplasm

Alanine, aspartate and glutamate metabolism, Nitrogen metabolism

Yes

  1. aDruggability predicted with DoGSiteScorer software. A druggability score above 0.60 is usually considered, but a score above 0.80 is favored [48].
  2. bMolecular weight was determined using ProtParam tool (http://web.expasy.org/protparam/).
  3. cMolecular function (MF) and biological process (BP) for each target protein was determined using UniProt.
  4. dCellular localization of pathogen targets was performed using CELLO.
  5. eKEGG was used to find the role of these targets in different cellular pathways.
  6. fPAIDB was used to check if the putative targets are involved in pathogen's virulence.