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Figure 4 | BMC Genomics

Figure 4

From: HOXD-AS1 is a novel lncRNA encoded in HOXD cluster and a marker of neuroblastoma progression revealed via integrative analysis of noncoding transcriptome

Figure 4

Up-regulation of HOXD-AS1 in SH-SY5Y neuroblastoma cell line by RA and stage correlation in patients. (A) SHSY5Y cells plated onto laminin coated dishes were induced to differentiate with 10 ┬ÁM retinoic acid (RA). Total RNA was extracted at the time-points of 0 hours (uninduced), 6, 24 and 120 hours. The amounts of the selected 13 probes were quantified by qRT -PCR using relative DDCt quantification method and expressed as a fold change relative to uninduced time-point (0 hours). (B) HOXD-AS1 expression correlates with neuroblastoma progression and differentiates between early (st. 1-2) and late (st. 3-4) stage tumors. Each patient of the whole studied population of 64 patients diagnosed with NB (55) is represented by a dot.

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