Figure 3From: Exome Sequencing of Normal and Isogenic Transformed Human Colonic Epithelial Cells (HCECs) Reveals Novel Genes Potentially Involved in the Early Stages of Colorectal TumorigenesisValidation of “deleterious” INCENP variants in ICT series. Predicted “deleterious” mutation p.M506T was confirmed by Sanger sequencing of PCR products from 1CT series. c. T1517>C mutation (highlighted in red box) was detected in all 1CT series as well as the pre-immortalized HCEC1 cells and it occurs at a highly conserved position.Back to article page