Results with simulated reads (length = 100 bps). d-accuracy (a) and d-amount (b) of wAve when integrating positions covered by more than one, two and three mappers. R err means read error rate. The red lines represent average d-accuracy or d-amount of the three mappers. (c) Detected amount of CpGs by Bismark, BSMAP, BS-seeker2, and wAve. For wAve, the considered positions are those covered by more than two mappers. (d) d-accuracy of methylation levels at CpG sites across read error rates. The d-accuracy was 1-difference between generated and detected methylation level at each block. The d-accuracy within all of hg19 chr19, LINEs, and SINEs, in cases that read error rate equals to 2% (e) and 8% (f). The d-accuracies in repeat regions were determined by evaluating d-accuracy of each block, in which more than half is covered by repeat regions. (g) Distribution of d-accuracies within 100 read sets, in which read conditions were varied in length (50 bps and 100 bps) and error rates (from 0% to 8%).