Figure 1
From: A pipeline for completing bacterial genomes using in silicoand wet lab approaches
The pipeline of genome assembling and gap closure. Clean data were subject to de novo assembling, initial gap filling, and single base corrections with SOAPdenovo and SOAPaligner; the resulting assembly was used for comparative genomics studies and also provided guidance for wet lab validations. Meanwhile, the clean data were assembled separately based on a reference genome using CLC Genomics Workbench. This second assembly was integrated into the first one to yield a hybrid assembly, which was then updated with public data, GapFish results, and Sanger sequencing data until the genome sequence was complete.