Figure 6

Co-expression network analyses. A) Using Cytoscape co-expressed genes are visualized as networks with nodes representing genes and edges reflecting pairwise co-expression relationships in healthy tissues. B) Numbers of co-regulated cancer genes in healthy tissues (right panel of the plot) are shown along with the mutation frequencies (left panel of the plot). Mutation frequencies are presented as stacked bars with cancer type dependent coloration. ERGs are sorted on the y-axis by the overall mutation frequencies. C) A subnetwork within the main co-expression network contains 24 co-expressed ERGs. Colors indicate the corresponding ERG families. D) EZH2 (green) and 99 co-expressed genes form one co-regulated network that is significantly enriched for cell cycle regulators. Genes that are directly connected with EZH2, because they show a very high degree of co-expression (R > 0.85), are highlighted in orange. Genes that are present in the network, but not directly connected with EZH2 are shown in blue. F) Examples of positive correlations between EZH2 and co-expressed cell cycle regulators. Each dot reflects the gene expression level (represented by variance stabilized RNAseq count data) of EZH2 (x axis) and the co-expressed gene (y axis). Dots are colored according to tissue type.