Figure 3From: High-throughput genotyping of single nucleotide polymorphisms with rolling circle amplificationEffect of 3' arm length and ligation temperature on allele discrimination. The 3' arm of the matching OCPs formed 12, 15, 20, or 23 basepairs, as indicated, when annealed to the oligonucleotide target. Ligation reaction temperatures were as indicated.Back to article page