Table I Sequence of oligonucleotides used in the study.
From: High-throughput genotyping of single nucleotide polymorphisms with rolling circle amplification
| Oligonucleotide | Sequence |
|---|---|
| OCP-1822inC | 5'GAAGAACTGGACAGATTTACTACGTGACTCGTCATGTCTCAGCTCTAGTA |
| CGCTGATCTTAGTGTCAGGATACGGAGATGTTCTGCTTTGTC | |
| OCP-1822ocT | 5'GAAGAACTGGACAGATTTACTACGTATGTTGACTGGTCACACGTCGTTCT |
| AGTACGCTTCTACTCCCTCTTGAGATGTTCTGCTTTGTT | |
| P1-1822inC- | 5'FAM-TCGATGACTGACGGTCATCG(DABCYL-dT)ACTAGAGCTGAGACA |
| FAM | TGACGAGTC |
| P1-1822ocT- | 5'TET-TCGATGACTGACGGTCATCG(DABCYL-dT)ACGACGTGTGACCAG |
| TET | TCAACAT |
| P2-1822inC | 5'aba-aba-GATACGGAGATGTTCTGCTTTGTC |
| P2-1822ocT | 5'aba-aba-CCTCTTGAGATGTTCTGCTTTGTT |
| OCP-1822C | 5'GAAGAACTGGACAGATTTACTACGTATTCTGACTCGTCATGTCTCAGCTT |
| TAGTTTAATACGACTCACATATCCCAGATGTTCTGCTTTGTC | |
| OCP-1822T | 5'GAAGAACTGGACAGATTTACTACGTATTCTGACTCGTCATGTCTCAGCTT |
| TAGTTTAATACGACTCACTATAGGGAGATGTTCTGCTTTGTT | |
| P2-1822C- | 5'TET-TCGATGACTGACGGTCATCG(DABCYL-dT)ATATCCCAGATGTTC |
| TET | TGCTTTGTC |
| P2-1822T- | 5'FAM-TCGATGACTGACGGTCATCG(DABCYL-dT)TATAGGGAGATGTTC |
| FAM | TGCTTTGTT |
| P1 | 5'aba-aba-CTAAAGCTGAGACATGACGAGTC |
| Oligonucleotides used in the studies of ligation | |
| kinetics, affect of OCP 3' arm length, and ligation | |
| temperature | |
| CFTR M1101K | 5'CTTGTACCTGTCAACACTGCGCTGGTTCCAAAXGAGAATAGAAATGATTT |
| target | T TGTCATCTT |
| x = T in wild type and A in mutant | |
| OCP-M1101K23 | 5'TTTGGAACCAGCGCAGTGTTGACACTCTGACTCGTCATGTCTCAGCTTTA |
| GTTTAATACGACTCACTATAGGGGACAAAAATCATTTCTATTCTCY | |
| OCP-M1101K20 | 5'TTTGGAACCAGCGCAGTGTTGACACTCTGACTCGTCATGTCTCAGCTTTA |
| GTTTAATACGACTCACTATAGGGAAAAATCATTTCTATTCTCY | |
| OCP-M1101K15 | 5'TTTGGAACCAGCGCAGTGTTGACACTCTGACTCGTCATGTCTCAGCTTTA |
| GTTTAATACGACTCACTATAGGGTCATTTCTATTCTCY | |
| OCP-M1101K23 | |
| 5'TTTGGAACCAGCGCAGTGTTGACACTCTGACTCGTCATGTCTCAGCTTTAG | |
| TTTAATACGACTCACTATAGGGTTTCTATTCTCY | |
| Y = A in wild type and T in mutant. The number 12, | |
| 15, 20 or 23 refers to the number of base pairs the | |
| 3' end forms with the target. |