Figure 4

Dendrogram of nucleophosmin/nucleoplasmin family members. Amino acid sequences from the 16 proteins shown were aligned with CLUSTAL W, and a dendrogram was produced using the TreeTop algorithm as described in Materials and Methods. The 16 sequences included 14 from the nucleophosmin/nucleoplasmin family (see Table 2 and Figure 3) and two other histone-binding proteins (NASP and N1/N2), which are closely related to each other but unrelated to the nucleophosmin/nucleoplasmin family. Bootstrap significance values are shown at the branchpoints for 100 resamplings. The analysis suggests that NPM3 is more related to nucleoplasmins than to nucleophosmins and that NPM3 is closely related to Xenopus NO29.