Immunoblot analysis of the cellular localization of HA-tagged NPM3. Protein samples (50 mg) were subjected to SDS-PAGE, electrophoretic transfer to nitrocellulose and immunoblotting with an anti-HA antibody. Media, cytoplasm and nucleus are the subcellular fractions analyzed. The locations of the molecular weight standards in kDa are indicated to the left of the blot. Arrows indicate bands associated with HA-tagged NPM3. (A) cells mock-transfected; (B) cells transfected with antisense HA-tagged NPM3 cDNA construct; (C) cells transfected with sense HA-tagged NPM3 cDNA construct.