Figure 4
From: Efficacy of SSH PCR in isolating differentially expressed genes
Enrichment of ratio differentially expressed φx174 DNA Hae III by SSH PCR. Fixed amount of 0.1% φx174 Hae III DNA was added in tester and various amount of φx174 Hae III DNA in driver, which resulted in serial five folds of dilutions. SSH PCR was carried out as described in Materials and Methods. Lane 1, φx174 Hae III DNA marker; lane 2, 0.1% of φx174 Hae III DNA in driver, lane 3, 0.02% of φx174 Hae III DNA in driver; lane 4, 0.004% of φx174 Hae III DNA in driver; lane 5, 0.0008% of φx174 Hae III DNA in driver; lane 6, 0.00016% of φx174 Hae III DNA in driver in driver; lane 7, no φx174 Hae III DNA in driver.