Isotyping of Kir expression in eosinophils, neutrophils and lung mast cells. Lung mast cells (A), eosinophils (B), and neutrophils (C) were purified and Kir isoform profiling performed according to the strategy described in the text. 50 bp DNA ladder was run as molecular weight standard with sizes indicated (bp). Potential correlation with Kir isoforms is indicated to the left of the appropriate fragment. *Fragment of unknown origin. Results are representative of two independent experiments. Gel images were scanned and processed using Adobe Photoshop Version 5.5.