Figure 3

Overview of the PCR promoter capture method A) Sheared genomic DNA of 4–10 Kb harboring the gene of interest (thick line) is used as template in B) asymmetrical PCR reactions. The reactions are conducted with a biotinylated high Tm anti-sense primer and C) the resulting single stranded fragments are purified with streptavidin coated magnetic particles. D) A secondary PCR reaction is conducted with an oligo dT₂₀ and a nested anti-sense primer.