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Table 1 Overview of experimental design

From: Cross-species hybridisation of pig RNA to human nylon microarrays

Filter:

1

2

3

4

5

6

3 rd Use

Human, 4

Pig, 2

Pig, 6

Pig, 4

Pig, 4

Pig, 4

4 th Use

Pig, 6

Human, 4

Pig, 2

Pig, 4

Pig, 4

Pig, 4

5 th Use

Pig, 2

Pig, 6

Human, 4

Pig, 4

Pig, 4

Pig, 4

  1. A total of 18 hybridizations were done on six nylon cDNA microarray filters (GF211; ResGen). Each filter was stripped and reused a total of five times, and these data are from the third, fourth and fifth use of each filter. Three replicates each of 4 μg human skeletal muscle RNA, and 2 and 6 μg of pig skeletal muscle RNA were hybridized to filters 1 through 3. A total of nine replicates of 4 μg of pig skeletal muscle RNA was hybridized to filters 4 through 6. Each entry shows the species and amount (μg) of total RNA used in the hybridization.