Figure 3

Scatterplot matrix using average expression ratios of multiple replicate amplifications for each protocol and the gold standard. The X-axis and Y-axis show virtual gene expression level [normalized log(base2) fluorescence intensity ratio of sample to reference averaged over multiple arrays] measured using aRNA amplified by different protocols or unamplified poly(A)⁺ RNA as labelled. The last column of plots shows each amplification protocol (Y-axis) vs. gold standard (X-axis). The correlation coefficient for each pair is listed in each plot. The orange and blue shaded regions indicate more than a two-fold difference between the virtual expression values for each protocol being compared.