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Table 2 Efficacy of amplification using 3 μg total RNA from BC91 and different ds cDNA cleanup methods, with or without TS primer.

From: Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis

Column used for ds cDNA cleanup

Bio-6

G-50

Amplification Protocol

TS

w/o TS

TS

w/o TS

Yield of aRNA (μg)

7.6 ± 1.0 (n = 5)

11.1 ± 2.2 (n = 3)

8.9 ± 3.8 (n = 6)

10.0 ± 5.1 (n = 6)

Fold of amplification

253 ± 33

370 ± 73

297 ± 126

333 ± 170