From: Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis
Column used for ds cDNA cleanup
Bio-6
G-50
Amplification Protocol
TS
w/o TS
Yield of aRNA (μg)
7.6 ± 1.0 (n = 5)
11.1 ± 2.2 (n = 3)
8.9 ± 3.8 (n = 6)
10.0 ± 5.1 (n = 6)
Fold of amplification
253 ± 33
370 ± 73
297 ± 126
333 ± 170