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Figure 2 | BMC Genomics

Figure 2

From: Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

Figure 2

Western blot analysis of the rat IAP proteins distribution in adult rat tissues. The protein samples prepared from fresh adult rat tissues, or bacterially expressed GST-fusion recombinant proteins, were separated on 10% SDS-PAGE (12 μg total protein per lane) and transferred onto a PVDF membrane using standard techniques [20]. Rabbit polyclonal antibodies against rat iap-1 (top), iap-2 (middle), and iap-3 (bottom) were used at 1:1,000 dilution followed by secondary antibody (horseradish peroxidase-conjugated goat anti-rabbit IgG; Amersham) used at 1:1,500 dilution. Antibody complexes were detected using the ECL system (Amersham). Position and size of the markers is indicated on the left. (Note that the recombinant GST-fusion proteins are larger due to the presence of N-terminal GST tag.) Tissues used are as follows: (1) brain, (2) heart, (3) intestine, (4) kidney, (5) liver, (6) lung, (7) spleen, (8) testis, and (9) thymus.

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