Table 2 The primer sequences and necessary concentrations for the quantification via real-time PCR.
product | forward primer | conc. | reverse primer | conc. |
|---|---|---|---|---|
GAPDH | ATCAACGGGAAGCCCATCA | 100 nM | GACATACTCAGCACCGGCCT | 100 nM |
MCP-2 | CTTCTCTGGGCTGACAGGGA | 300 nM | TCTACGCAGTGCTTCTTTGCC | 300 nM |
cystatin C | CAAGAAGAGTGGAGCCAGGG | 50 nM | GCAGGCAGGTTCTGCACAT | 50 nM |