Skip to main content

Table 3 Parameter estimates for the total data set consisting of 10759 genes and for the "conservative set" of 2721 genes.

From: Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance

Parameter

Type of effect1

Estimate Total set

Estimate Conserved set

μ

Fixed

10.56

12.18

C (cell line 2; U2OS)

Fixed

0.086

-0.028

D (Cy3)

Fixed

-0.026

-0.022

P (non-amplified)

Fixed

-0.74

-1.14

CP22 (cell line 2 and non-amplified)

Fixed

-0.0043

0.0089

σ A (array)

Random

0.28

0.30

σ B (batch)

Random

0.041

0.041

σ C (gene)

Random

1.36

0.98

σ AD

Random

0.039

0.040

σ GP

Random

0.85

0.79

σ CG

Random

0.74

0.54

σ AG

Random

0.44

0.44

σ DG

Random

0.048

0.050

σ CGP

Random

0.22

0.028

σε

Random

0.22

0.22

  1. Annotations: μ = overall mean signal intensity, C = cell line type, D = labeled dye, P = protocol. 1 Of the fixed effects, C, D, and P have two levels while CP is an interaction between two fixed effects. The random effects were modeled as independently normally distributed random effects with zero mean and constant standard deviation. σ CGP is the cell line specific GP effect and is the effect of primary interest.