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Table 1 Primers pairs used for RT-PCR analysis of T versusTWistranscripts. See Materials and Methods for primer sequences.

From: Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus

Forward primer

Reverse primer

Product Size (in bp)

Name

Location1

Name

Location1

 

F1

T exon 6

R1

T exon 8

308

F2

T exon 4

R1

T exon 8

523

F1

T exon 6

BGH037

ETn element

2062

F3

T exon 7, last 35 bp

R1

T exon 8

161

  1. 1 Genomic organization of T locus from [10, 16], and Bernhard G. Herrmann personal communication. 2 Assuming proper T exon 6 to 7 splicing, then read-through transcription from exon 7 into adjacent ETn sequences, in TWisallele transcripts only.
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BMC Genomics

ISSN: 1471-2164

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