Table 2 PCR primer sequences
From: Partial duplication of the APBA2 gene in chromosome 15q13 corresponds to duplicon structures
Exon | Exon Size (bp) |  | Primers | Ta (°C) | Product Size (bp) |
|---|---|---|---|---|---|
1 | 117 | F | 5'-GAGCGTGTGGCCCATCGTT-3' | 57 | 291 |
| Â | Â | R | 5'-AGCACATCAGCCCAGACTCATTCA-3' | Â | Â |
2 | 53 | F | 5'-CATTTTTATACTTGAAGGAGCTCT-3' | 50 | 163 |
| Â | Â | R | 5'-GCTGCCTTGTATTTCTTACCTAA-3' | Â | Â |
3 | 991 | F | 5'-TGTGGGGTTTTGACTCTGTCCTTCC-3' | 60 | 1098 |
| Â | Â | R | 5'-GTACCCTGAGGTCCCTTGCTCTCG-3' | Â | Â |
4 | 83 | F | 5'-CTTTAACTTCCCCGTTTCCAGGTTTG-3' | 57 | 137 |
| Â | Â | R | 5'-TCCAGCCCAGAACTCTCATCCTTGAA-3' | Â | Â |
5 | 38 | F | 5'-GCATATTCAGGTTTTGCATCTTGC-3' | 54 | 185 |
| Â | Â | R | 5'-CAGCCCAGAACTCTCATCCTTGAA-3' | Â | Â |
6 | 146 | F | 5'-CCTGGCTGCCGTGTTCCTTGTG-3' | 61 | 316 |
| Â | Â | R | 5'-CGCCATATTCCCTCCTGCTAGTGC-3' | Â | Â |
7 | 34 | F | 5'-AGACCTGAGAGTGGGGGCATCAAC-3' | 57 | 276 |
| Â | Â | R | 5'-TCACCTACCAAGGGAAGAGGAAAAGC-3' | Â | Â |
8 | 88 | F | 5'-TCTGGTGATTAGGGATGTTGAGCA-3' | 55 | 259 |
| Â | Â | R | 5'-ATACCATGGGACAAGAAGGAGTCG-3' | Â | Â |
9 | 189 | F | 5'-CCACCATGCCCAGCCCTGGAGTAC-3' | 64 | 313 |
| Â | Â | R | 5'-GCTGTGAACTTTGGGAGGGGAGTGC-3' | Â | Â |
10 | 179 | F | 5'-CCTGTGCCACGTGCCTGTCTCCAG-3' | 62 | 253 |
| Â | Â | R | 5'-CCTCCCTGAGGCTGGTGGGTGTGG-3' | Â | Â |
11 | 215 | F | 5'-TCCTCCTGTGGGTCCTTGGCAGAG-3' | 64 | 345 |
| Â | Â | R | 5'-GGGGATGAAGTGAGGCAGGGTTGA-3' | Â | Â |
12 | 121 | F | 5-TAAAGCCCAGCCTGTGACTCCTGTCC-3' | 62 | 251 |
| Â | Â | R | 5'-CCAGACATAGCCTGCCCCATTGGA-3' | Â | Â |
13 | 140 | F | 5'-CTGTCGGGTGTGGCGGGAACACGT-3' | 64 | 275 |
| Â | Â | R | 5'-CTCTGGAGCGGGGGAGGCTCATGG-3' | Â | Â |
14 | 1251 | F | 5'-GCGGCTCAGCCTCCTGTTTCTGTC-3' | 62 | 200 |
| Â | Â | R | 5'-CTGTGGCGTCGGGGTCAAGTCTGC-3' | Â | Â |