Skip to main content

Table 2 PCR primer sequences

From: Partial duplication of the APBA2 gene in chromosome 15q13 corresponds to duplicon structures

Exon Exon Size (bp)   Primers Ta (°C) Product Size (bp)
1 117 F 5'-GAGCGTGTGGCCCATCGTT-3' 57 291
   R 5'-AGCACATCAGCCCAGACTCATTCA-3'   
2 53 F 5'-CATTTTTATACTTGAAGGAGCTCT-3' 50 163
   R 5'-GCTGCCTTGTATTTCTTACCTAA-3'   
3 991 F 5'-TGTGGGGTTTTGACTCTGTCCTTCC-3' 60 1098
   R 5'-GTACCCTGAGGTCCCTTGCTCTCG-3'   
4 83 F 5'-CTTTAACTTCCCCGTTTCCAGGTTTG-3' 57 137
   R 5'-TCCAGCCCAGAACTCTCATCCTTGAA-3'   
5 38 F 5'-GCATATTCAGGTTTTGCATCTTGC-3' 54 185
   R 5'-CAGCCCAGAACTCTCATCCTTGAA-3'   
6 146 F 5'-CCTGGCTGCCGTGTTCCTTGTG-3' 61 316
   R 5'-CGCCATATTCCCTCCTGCTAGTGC-3'   
7 34 F 5'-AGACCTGAGAGTGGGGGCATCAAC-3' 57 276
   R 5'-TCACCTACCAAGGGAAGAGGAAAAGC-3'   
8 88 F 5'-TCTGGTGATTAGGGATGTTGAGCA-3' 55 259
   R 5'-ATACCATGGGACAAGAAGGAGTCG-3'   
9 189 F 5'-CCACCATGCCCAGCCCTGGAGTAC-3' 64 313
   R 5'-GCTGTGAACTTTGGGAGGGGAGTGC-3'   
10 179 F 5'-CCTGTGCCACGTGCCTGTCTCCAG-3' 62 253
   R 5'-CCTCCCTGAGGCTGGTGGGTGTGG-3'   
11 215 F 5'-TCCTCCTGTGGGTCCTTGGCAGAG-3' 64 345
   R 5'-GGGGATGAAGTGAGGCAGGGTTGA-3'   
12 121 F 5-TAAAGCCCAGCCTGTGACTCCTGTCC-3' 62 251
   R 5'-CCAGACATAGCCTGCCCCATTGGA-3'   
13 140 F 5'-CTGTCGGGTGTGGCGGGAACACGT-3' 64 275
   R 5'-CTCTGGAGCGGGGGAGGCTCATGG-3'   
14 1251 F 5'-GCGGCTCAGCCTCCTGTTTCTGTC-3' 62 200
   R 5'-CTGTGGCGTCGGGGTCAAGTCTGC-3'   
  1. Forward (F) and reverse (R) oligonucleotide sequences are shown along with annealing temperatures (Ta) and PCR product size