Skip to main content

Table 2 Primers used in this study

From: A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast

Primer

Sequence

Restriction site

Group A*

  

ADH-F

5'-atgcgggccc CCTACAACAACTAAGAAAATGGC-3'

Apa 1

ADH-R

5'-atgcatgcctcgag CCACTATTTATACCATGGGAGG-3'

Xho 1

URA-ARS-F

5'-atgcatcgat GTCGAAAGTTACATATAAGGAACGT-3'

Cla 1

URA-ARS-R

5'-atgcggatcc CAGTTTTTTAGTTTTGCTGGCCGC-3'

Bam H1

alu45-F

5'-atgcatcgat GTCCCAGCTACTTGGGAGGC-3'

Cla 1

alu45-R

5'-atgcatgcctcgag GGAGTGCAGTGGCGGGATCT-3'

Xho 1

alu65-F

5'-atgcatcgat CGTAGTGGCGGGCGCCTGTA-3'

Cla 1

alu65-R

5'-atgcatgcctcgag TCTCGCTCTGTCGCCCAGGC-3'

Xho 1

alu75-F

5'-atgcatcgat ATTAGCCGGGCGTAGTGGCG-3'

Cla 1

alu75-R

5'-atgcatgcctcgag GAGACGGAGTCTCGCTCTGT-3'

Xho 1

alu190-F

5'-atgcatcgat CTAAAACGGTGAAACCCCGT-3'

Cla 1

SV-ARS-F

5'-atgcatcgat TTATACACTTAAAAATTTTA-3'

Cla1

SV-ARS-R

5'-atgcatgcctcgag CTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCGCCACCt TGCCCG-3'

Xho 1

HP-ARS-F1

5'-atgcatgcctcgag CCTCGGCTTCTTCTGGGA-3'

Xho 1

HP-ARS-R1

5' atgcatgcgtcgac GTTTGCAGGCTCACTAGGTAG 3'

SalI

HP-ARS-F2

5' atgcatgcgtcgac GTAGGATATGCCCTTGACTAT 3'

SalI

HP-ARS-R2

5'-atgcatcgat GAAGCAATTACTTACATTCAA-3'

Cla 1

ALF-F

5'-atgcatcgat AAGAGTGTTTCAAAACTGCT-3'

Cla 1

ALF-R

5'-atgcatgcctcgag CATTCTCAGAAACTTCTTTGTGAa GTTTGC-3'

Xho 1

Group B*

  

ALU-DET-F

5'-GGCCGGGCGCGGTGGCTCACGCCTGTAA-3'

 

ALU-DET-R

5'-TGTATTTTTAGTAGAGACGGG-3'

 

Y-SAT-F

5'-GAATCTGCAAGTGGATATTTG-3'

 

Y-SAT-R

5'-TCTGTGAGATGAATGCACACA-3'

 
  1. Upper case letters indicate homologous regions. Lower case letters indicate nonhomoloqous sequences. Bold letters indicate endonuclease restriction sites used for cloning PCR products. Underlined lower case letters indicate nucleotide substitutions introduced into the primer to eliminate an out-of-frame ATG initiation codon in the targeting hook. *Group A includes primers used for plasmid construction; group B includes primers used for Alu- and Sat-probes.