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Table 1 Mass yields generated by IVT from varying amounts of restricted DNA.

From: Development and validation of a T7 based linear amplification for genomic DNA

Input (ng)

Yield (ug)

n-fold amp*

50.0

78.7

1574

25.0

69.8

2790

10.0

54.2

5420

5.0

25.8

5163

2.5

10.3

4114

50.0 PCR

15

300

  1. *Yield and fold amplification may be overestimated for low starting concentration due to non-specific or template independent products (see text). The yield for the PCR amplification could be improved by introducing more cycles. However, this would likely increase bias.
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BMC Genomics

ISSN: 1471-2164

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