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Table 1 Mass yields generated by IVT from varying amounts of restricted DNA.

From: Development and validation of a T7 based linear amplification for genomic DNA

Input (ng) Yield (ug) n-fold amp*
50.0 78.7 1574
25.0 69.8 2790
10.0 54.2 5420
5.0 25.8 5163
2.5 10.3 4114
50.0 PCR 15 300
  1. *Yield and fold amplification may be overestimated for low starting concentration due to non-specific or template independent products (see text). The yield for the PCR amplification could be improved by introducing more cycles. However, this would likely increase bias.
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BMC Genomics

ISSN: 1471-2164

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