A high resolution physical and RH map of pig chromosome 6q1.2 and comparative analysis with human chromosome 19q13.1

Martins-Wess, Flávia; Milan, Denis; Drögemüller, Cord; Voβ-Nemitz, Rodja; Brenig, Bertram; Robic, Annie; Yerle, Martine; Leeb, Tosso

doi:10.1186/1471-2164-4-20

Table 1 Primer sequences for RH mapping

From: A high resolution physical and RH map of pig chromosome 6q1.2 and comparative analysis with human chromosome 19q13.1

Primer	Forward sequence (5 '-3')	Reverse sequence (5 '-3')	Tm (°C)	PCR-Product (bp)	Retention IMpRH	Retention IMNpRH2
BLVRB	GGT TAT GAG GTG ACA GTG	CCA AGA GCA CGA TGA TGG	55	148	0.14	0.38
CAPNSl	TGT ATT CCT GAA CGG GAG	TGC AAG AGA GGG CTA ATG	54	272	0.31	0.38
COX7A1	CAC CTA CTG GAC GAA TCC	AGG TCC CGA GGT ATT ACA G	59	388	0.29	0.38
DLL3	ACC CCA GAA TCC TCG TAC	AGA GCA GGA CAT AGC ATC	55	395		0.39
ECHl	ACC AGG AGG TCC TGC TTG	ATG TAG TTG AGG CCC TCT G	58	136	0.18	0.34
GRIK5	CAA CTT CCA GGC CCT GTC	TTG ATG AGC TCG CCA ACC	60	174		0.31
ITZ001	TAG CCT TTC CTG TGG AGG	ACA CAA AAG CAC ACA CCG	56	423	0.19	0.32
ITZ002	AGC CTT CAC CCA GAA GTC	GAT GCA CAA GGA GCT GAG	59	287		0.36
ITZ003	CAT AGG GCT TTT CAC CAG	TTC TTG CCG AAT ATC AGA G	54	297		0.28
ITZ004	TTC ACT TTG GGT CTG CTG	ACC TAC CCA CTG CTA TGC	55	311	0.24	0.26
ITZ005	TCC AGA CAA GTG AGA AAC AA	TTA ATT TAT AAT GCC TGG TCA	55	320	0.24	0.37
ITZ006	ATC TGC GGA GAG GAA AAG	TCG TGT TTG TTG GAA TGT C	54	201		0.35
ITZ007	GTG ACT TGT AGA CCA CAG	CCT ACA GAG GGA GAA TCC	55	346		0.35
ITZ008	AGC TGA GAC CAA TGC CAT	ATA ATT GGG AGT TCT CGC	53	122		0.36
ITZ009	CTG CTC CTC ATT CCC ATG	CCG TCT TAT GCT TGA GTC	55	187		0.40
ITZ010	CAG ATT GGG ATG AAG CTC	TTA TCA ATC CCA ACA CAC C	54	114	0.22	0.37
ITZ011	AGA CGG GAA ATT GAG ACC	TCC CTG TGG CAG TAA ATG	58	345		0.37
ITZ012	GTG GGG CCC TAT AAA GAC	CCG AGG GTC AAA TGT CTG	56	223		0.38
ITZ013	TTG GAG GGT TCA ACT ACG	GTA AAC CCG TTC ACG TTG	57	197		0.38
ITZ014	TGC CTG TTC ACG AAC CAC	TCC TTG TGT GGG CTA CAG	59	100		0.32
ITZ015	AAG AAA AGG AAA AGG TTT GG	TGT TCT CGC AAA CAG TGA G	58	304		0.33
ITZ016	AGA CAG GCT CCG ATG AAG	AGA AAG GCT TCC CTG CTG	59	209		0.35
LGALS4	GAT GTC GCC TTC CAC TTC	TGA TGA CCA GCT CGA AGG	56	142	0.18	0.35
LOC 163128	TCC AAA GAG AAG GTG GTG	ATG AGG GCA TAG GAG AGC	55	170		0.36
DKFZp434H247	TTC TGC AGC TTC TTC TGC	ATC GAA GTC CTG TTG CTG	54	145		0.41
MAP4K1	CCT ACC CAC GCC TAT GC	CCA GCC AGC AGG AAA GC	56	136		0.33
NFKBIB	CTG CAC CTG GCA GCC ATC	GCT GGA GCA GCA CGC AAG	60	154		0.32
PAK4	CAG CGA GTG TCC CAT GAG	CAT GGG TCA GCA GGA TGG	58	~1600	0.19
POLR21	ACC ACC CGT GCC AAA AG	CCG CGC ACT GTG TGA CT	55	172		0.37
PPP1R14	GCT GAG CAA GCT GCA GTC	GGT ACA GCT CCT CCA AGC	58	164	0.32
PRX	GCC TCA GGT GAC CTT GTC	CCC ACA TCC AGC TCA AGC	58	117	0.21	0.38
RBTl	CCA TGG ATG AGA CTG AGC	GGC ACA GAA GAG GTT GTG	56	218		0.33
RPS16	CGC TGA TCA TCA CGA TGG	GCT TTT GGG CAA GGA ACG	56	294	0.24	0.41
RYR103/104 (RYR_Promoter)	TTC GTT TCT GCT TCG CC	CTC TCT CCT CCC ATT TC	48	162	0.20	0.34
SEIl	GAG CTG GAT GAT GCT GAG	GCT GTG ATG GAG CTT GAG	56	184		0.32
SPTBN4	ATA TCC TGC CCC AAG AAG	GAG GAG GTC GAC GTT TTG	58	282	0.20	0.38
SUPT5H	AGG AGC TTC CCC AGG AAG	TGG GTG AGG ATC GGG AAG	58	121	0.18
SW193	TGC CAT CCT TTC TTT CAT TAC G	TCA CTC TGA GGG GTC CTG AC	62	101		0.37

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ISSN: 1471-2164

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