Table 1 DNA sequences
From: High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay
Open Circle Probes | Length | Nucleotide Sequence |
|---|---|---|
FV Normal | 78 bases | 5'-GCCTGTCCAGGGATCTGCTTCTTCGGTGCCCATCGCGCAGACACGATA GAGGAATACAACAAAATACCTGTATTCCTC-3' |
FV WT-no hairpin | 68 bases | 5'-GCCTGTCCAGGGATCTGCTTCTTCGGTGCCCATCGCGCAGACACGATA ACAAAATACCTGTATTCCTC-3' |
FV Mutant | 78 bases | 5'-GCCTGTCC AGGGATCTGCTCTGTTATCGGCCGTCGCGCAGACACGATA AAGGAATACAACAAAATACCTGTATTCCTT-3' |
FV Mut-no hairpin | 68 bases | 5'-GCCTGTCCAGGGATCTGCTCTGTTATCGGCCGTCGCGCAGACACGATA ACAAAATACCTGTATTCCTT-3' |
FII Normal | 79 bases | 5'-agcctcaatgctcccagtgcACAAGACCGAAAGGGTAGTCGCGGATTGTTG CGCTGAGAaataaaagtgactctcagcg-3' |
FII Mutant | 79 bases | 5'-agcctcaatgctcccagtgcACTCAATCCCAGGCGAGTCGCGGATTGTTG TGCTGAGAGaataaaagtgactctcagca-3' |
H63 Normal | 99 bases | 5'-ATCATAGAACACGAACAGCTGGTAGTATTGATCGGCCACTGGTATCTCGTCCG TGCTAGAAGGAAACATGAGAGTAGGCTCCACACGGCGACTCTCATG-3' |
H63 WT-no hairpin | 99 bases | 5'-ATCATAGAACACGAACAGCTGGTAGTATTGATCGGCCACTGGTATCTCGTCCG TGCTAGAAGGAAATCAAGAGATGGGCTCCACACGGCGACTCTCATG-3' |
H63 Mutant | 99 bases | 5'-ATCATAGAACACGAACAGCTGGTAATGTTGACTGGTCACACGTCGTATCGTCCG TGCTAGAAGGAAAGATGAGAGTCAGGCTCCACACGGCGACTCTCATC-3' |
H63 Mut-no hairpin | 99 bases | 5'-ATCATAGAACACGAACAGCTGGTAGTATTGATCGGCCACTGGTATCTCGTCCG TGCTAGAAGGAAATCAAGAGATGGGCTCCACACGGCGACTCTCATC |
C282 Normal | 89 bases | 5'-CCAGGTGGAGCACCCAGGTCAGTTATCGGGCCGAGGCGCGAGTGTAAA CACGTATATCTCTCCCCTGGGGAAGAGCAGAGATATACGTG-3' |
C282 Mutant | 90 bases | 5'-CCAGGTGGAGCACCCAGGTTCTACCTCAGCCCGAAGGCGCGAGTGTAAA CACGTATATCTCTCCCCTGGGGAAGAGCAGAGATATACGTA-3' |
Primers | Length | Nucleotide Sequence |
FV Normal P1 | 31 bases | 5'-/56-FAM/ TCATTCGCAATCAATGGGCACCGAAGAAGCA-3' |
FV Mutant P1 | 31 bases | 5'-/5Cy3/TCATTCGCAATCAACGGCCGATAACAGAGCA-3' |
FV P2 | 15 bases | 5'-CGCGCA GACACGATA-3' |
FV 5' allele specific primer | 18 bases | 5'-GAGGAATACAACAAAATA-3' |
FV 3' allele specific primer | 15 bases | 5'-GATCCCTGGACAGGC-3' |
FII Normal P1 | 28 bases | 5'-/56-FAM/ TCATTCGCAATCACCCTTTCGGTCTTGT-3' |
FII Mutant P1 | 28 bases | 5'-/5Cy3/ TCATTCGCAATCACGCCTGGGATTGAGT-3' |
FII P2 | 15 bases | 5'-AGTCGCGGATTGTTG-3' |
FII 5' allele specific primer | 15 bases | 5'-CACTGGGAGCATTGA-3' |
H63 Normal P1 | 29 bases | 5'-/56-FAM/ TCATTCGCAATCAGATACCAGTGGCCGAT-3' |
H63D Mutant P1 | 27 bases | 5'-/5Cy3/ TCATTCGCAATCAACGACGTGTGACCA-3' |
H63 P2 | 18 bases | 5'-GTCCGTGCTAGAAGGAAA-3' |
H63 5' allele specific primer | 17 bases | 5'-TGTTCGTGTTCTATGAT-3' |
H63 3' allele specific primer | 15 bases | 5'-GGCTCCACACGGCGA-3' |
C282 Normal P1 | 28 bases | 5'-/56-FAM/ TCATTCGCAATCACGGCCCGATAACTGA-3' |
C282Y Mutant P1 | 28 bases | 5'-/5Cy3/ TCATTCGCAATCA CGGGCTGAGGTAGAA-3' |
C282 P2 | 15 bases | 5'-AGGCGCGAGTGTAAA-3' |
C282 5' allele specific primer | 15 bases | 5'-GGGTGCTCCACCTGG-3' |
C282 3' allele specific primer | 14 bases | 5'-GCAGAGATATACGT-3' |