Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs

Table 2 Putative novel genes identified by sequencing of random hamster testis cDNAs

Clone number¹	Size (bp)²	Accession Number³	Human ⁴	Mouse⁵	Rat⁶	Human cDNA⁷	Macaque cDNA⁷	Mouse cDNA⁷	Rat cDNA⁷	Fugu cDNA⁷
1030	241	BI431001	9q21.32	13B2	17	AL133654	AB072777 AB070138	Cons	Cons	ND
7012	221	BI431002	5p12	15A1	2	XM_059659	AB070167	Cons	Cons	ND
9004	264	BI431003	1p13.3	3F3	2	AL832216 AK095517 XM_086235	AB074456	ND	ND	ND
13043	169	BI431004	3q13.2	16B5	11	XM_120088	AB070087	XM_156183	ND	ND
15014	205	BI431005	11q21	9A2	8	AK058122 BC017929	AB070180	XM_134746	ND	ND
15018	142	BI431006	4q28.1	3B	2	NM_144643 AK093361	ND	AK011907	NM_153740	ND
15037	178	BI431007	10q26.11	19D2	1	AL834368	ND	XM_140750	ND	ND
19045	212	BI431008	1q32.3	1H6	13	NM_020197	ND	XM_129660	Cons	Cons

The eight cDNAs that detected specific signals in Northern analysis were analyzed further. Key: 1: Clone number refers to the position of the particular clone in a 96 well plate, i.e. 1030 is clone 30 on plate 1. 2: Size of original hamster cDNA insert in base pairs (bp). 3: Each sequence has been submitted to dbEST at NCBI. 4, 5, 6: The location on the human. Mouse or rat genome sequences that displayed the most significant match to the hamster sequence. 7: Accession numbers for orthologous cDNAs in public databases. ND: not detected. Cons: constructed in this study.

ISSN: 1471-2164