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Figure 1 | BMC Genomics

Figure 1

From: Whole-genome microarrays of fission yeast: characteristics, accuracy, reproducibility, and processing of array data

Figure 1

Effects of array element size and position on fluorescence signals and ratios. (A) PCR products of varying sizes (80–1500 bp) were used as array elements for two genes (mid1 and cdc12). In all cases, the 3' ends of the array elements were kept constant (~50 bp upstream of the stop codon). Top: fluorescence signals (local background subtracted) relative to array element size; the means and standard deviations of eight signal measurements are shown (two self-self experiments with two replicate measurements of both Cy3 and Cy5 each). Bottom: normalized ratios of signals (Cy5/Cy3) relative to array element size; the means and standard deviations of four measurements are shown (two self-self experiments with two replicate measurements each). (B) PCR products from varying positions within two genes (ags1 and tif471) were used as array elements. In all cases, the sizes of array elements were similar (~500 bp). Top: fluorescence signals (local background subtracted) relative to array element position (measured as distance of 3'ends of elements to stop codon); the means and standard deviations of eight signal measurements are shown (two self-self experiments with two replicate measurements of both Cy3 and Cy5 each). Bottom: normalized ratios of signals (Cy5/Cy3) relative to array element position; the means and standard deviations of four measurements are shown (two self-self experiments with two replicate measurements each).

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