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Table 3 Reproducibility of array data

From: Whole-genome microarrays of fission yeast: characteristics, accuracy, reproducibility, and processing of array data

Measurement

Mean SD (Range) a)

CV (Range) b)

Within array replicates c)

0.04 (0.03–0.06)

4.4% (3.1–6.2%)

Technical repeats d)

0.04 (0.02–0.06)

4.5% (2.5–6.3%)

Biological repeats e)

0.07 (0.05–0.10)

6.4% (4.9–8.1%)

  1. a) Standard deviation (SD) of signal ratios were calculated for each measurable pair of array elements, and the mean SDs of all repeated measurements are indicated. The range indicates the lowest and highest mean SDs obtained from several pairs of comparisons. b) CV: Coefficient of variation: [(SD of signal ratios × 100)/mean of signal ratios] was calculated for each measurable pair of array elements, and the mean CVs of all repeated measurements are indicated. The range indicates the lowest and highest mean CVs obtained from several pairs of comparisons. c) Determined from 10 arrays of experiments used in this study (4 self-self, 1 temperature, 4 media, and 1 harvesting experiment; see Methods). d) Determined from 7 pairs of arrays from this study (4 self-self, 4 media) and from [17](oxidative stress); for each pair identical RNA samples were used that were labelled independently and with reverse colors for the two hybridisations. Within array replicate data were averaged before analysis. e) Determined from 9 pairs of arrays from this study (4 self-self, 4 media) and from [17](oxidative stress); for each pair RNA samples from identical but independent biological experiments were used that were labelled with reverse colors for the two hybridisations. Within array replicate data were averaged before analysis.
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BMC Genomics

ISSN: 1471-2164

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