Table 3 Primer sequences and PCR conditions for amplification of epsilon class GST genes by quantitative PCR.
Gene | Forward Primer (5' to 3') | Reverse Primer (5' to 3') | cDNA amplicon (bp) | gDNA amplicon (bp) | Annealing/Detection Temp (°C) |
|---|---|---|---|---|---|
GSTe1 | GTCAATGAGCCACTGCACTTC | GTGATCCGGCTACGTAATCG | 175 | 253 | 57/84 |
GSTe2 | ATCACCGAGAGCCACGCAATCAT | GCCACCGTTCGCTTCCTCGTAGT | 414 | 507 | 62/84 |
GSTe3 | GAGCTGACGGCAAAGATGATCG | CCTGCTTCACTAGATCCTTCGC | 229 | 298 | 61/83 |
GSTe4 | CGCCATTCAAACGACCATGCC | GATGGCGTGGCTGTCCCACACG | 229 | 229 | 62/85 |
GSTe5 | ATGGCAACGAACCCCATCATC | CACACCACCATCGTCAATCACC | 198 | 198 | 62/85 |
GSTe6 | GTACACGCACACGATTAGTCC | GCTGACCCTTGAAGACGTTC | 104 | 195 | 62/83 |
GSTe8 | GCCATGATTCTGTACTACGACG | GGTAAGCGTTAACTCACCGTG | 198 | 267 | 63/83 |
GSTe7 | GCAGATTGGTACTGTACACG | CTCGGATAGAGACCGTCGTC | 256 | 335 | 59/84 |
S7 | GCACGTCGTGTTCATTGCCG | GAACGTAACGTCACGGCCAGTCA | 292 | 441 | 60/86 |