Figure 1From: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coliSequence of the polylinker. The polylinker for pB42-C1 is shown. Restriction sites are underlined and labeled. The sequence of pB42-C2 and pB42-C3 are identical except for the addition of one and two additional G residues immediately prior to the EcoRI site, as indicated.Back to article page