Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli

Table 1 Plasmid Loss During Growth in Rich Media

Plasmid	Media	Plate	Colonies	Percent lost
pB42	YPD	YPD	112	13
		SD-Trp	97
	YPGal	YPD	127	13
		SD-Trp	110
pB42-ftsZ	YPD	YPD	126	8
		SD-Trp	116
	YPGal	YPD	98	92
		SD-Trp	8

Yeast strain YM4271 transformed with the plamids indicated in the table were grown for 5 hours in YPD or YPGal (as indicated) and plated as serial dilutions on both YPD and SD-Trp. Colonies growing from these cultures following 100 μl of a 1:10000 dilution were counted. Data from this experiment is representative of four separate experiments.

ISSN: 1471-2164