Skip to main content

Table 2 Analysis of Yeast Two-Hybrid Libraries to the E. coli and B. subtilis genomes.

From: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli

Pool Vector Digest Insert digest Site frequency (%) Predicted number of 5' ends Transformants Transformants/vector F1 Expected number of 5' ends missing % coverage / pool
E1 EcoRI Tsp509I 0.306 35728 1,560,000 520,000 4.78 E-07 0 100
E2 ClaI MspI 0.511 55200 240,000 80,000 0.238 12957 77
E3 ClaI MaeII 0.403 30360 230,000 76,667 0.088 2430 92
E4 ClaI TaqαI 0.325 35728 230,000 76,667 0.117 4179 88
E5 BglII Sau3A 0.416 35728 1,310,000 436,667 4.92 E-06 0 100
B1 EcoRI Tsp509I 0.655 55232 998,000 332,667 0.00242 134 100
B2 ClaI MspI 0.38 32034 165,000 55,000 0.180 5754 82
B3 ClaI MaeII 0.237 19986 260,000 86,667 0.013 261 99
B4 ClaI TaqαI 0.286 24028 222,000 74,000 0.0460 1105 95
B5 BglII Sau3A 0.472 39762 1,320,000 440,000 1.56 E-05 1 100
  1. Calculations of site frequencies and prediction of the number of 5' ends are based on published genome sequences.