Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli

Table 2 Analysis of Yeast Two-Hybrid Libraries to the E. coli and B. subtilis genomes.

Pool	Vector Digest	Insert digest	Site frequency (%)	Predicted number of 5' ends	Transformants	Transformants/vector	F1	Expected number of 5' ends missing	% coverage / pool
E1	EcoRI	Tsp509I	0.306	35728	1,560,000	520,000	4.78 E-07	0	100
E2	ClaI	MspI	0.511	55200	240,000	80,000	0.238	12957	77
E3	ClaI	MaeII	0.403	30360	230,000	76,667	0.088	2430	92
E4	ClaI	TaqαI	0.325	35728	230,000	76,667	0.117	4179	88
E5	BglII	Sau3A	0.416	35728	1,310,000	436,667	4.92 E-06	0	100
B1	EcoRI	Tsp509I	0.655	55232	998,000	332,667	0.00242	134	100
B2	ClaI	MspI	0.38	32034	165,000	55,000	0.180	5754	82
B3	ClaI	MaeII	0.237	19986	260,000	86,667	0.013	261	99
B4	ClaI	TaqαI	0.286	24028	222,000	74,000	0.0460	1105	95
B5	BglII	Sau3A	0.472	39762	1,320,000	440,000	1.56 E-05	1	100

Calculations of site frequencies and prediction of the number of 5' ends are based on published genome sequences.

ISSN: 1471-2164