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Table 2 Comparison of hybridization signals between different batches (Batch800, Batch400, and Batch616) of arrays.

From: A novel design of whole-genome microarray probes for Saccharomyces cerevisiae which minimizes cross-hybridization

 

Total number of arrays

Median

Mean

  

Background

Probe

Background

Probe

TA

Cy5 Channel

      

Batch800

2

50

536

57

1379

24.0

Batch400

5

314

681

335

1993

5.9

Batch616

13

54

1570

72

3761

52.2

Cy3 channel

      

Batch800

2

56

506

62

971

15.6

Batch400

5

249

1047

293

3092

10.5

Batch616

13

75

1144

92

2627

28.5

  1. For all arrays of a given batch, the mean intensity value for each gene was first calculated considering all intensity measurements above 95% of the background threshold. Distribution of intensities were computed for all genes. Results are described by their medians and means. The transcript abundance (TA) is the mean intensity of the probe in the batch over mean intensity of the background signal.