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Table 6 Amplified samples show differential loss of 5' sequences.

From: Reproducibility of oligonucleotide arrays using small samples

Ā 

Mean log2ratio

Target range

Probe set

0.1 Ī¼g

0.5 Ī¼g

(nt from 3')

GAPDH 5'

-3.1

-3.1

883ā€“1153

GAPDH M

-2.6

-2.7

541ā€“835

GAPDH 3'

-1.6

-1.6

210ā€“492

Ī²-actin 5'

-3.2

-3.7

798ā€“1116

Ī²-actin M

-2.5

-2.6

432ā€“726

Ī²-actin 3'

-1.0

-1.3

0ā€“390

  1. Average log2 ratios of expression in the amplified samples to that in the control sample (that started with 10 Ī¼g RNA) from the same animal. Target range is the position of the target sequence from the 3' end of the transcript.
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BMC Genomics

ISSN: 1471-2164

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