Figure 2

Chromosomal localisation and gene structure of the human Sp7 gene. (A) Sp7 localises to 12q13 and the genes neighbouring Sp7 are the TAR (HIV) RNA binding protein 2, TARBP2; Specificity protein 1, Sp1; Specificity protein 7, Sp7; Aladin, Achalasia, adrenocortical insufficiency gene, AAAS; ATP5G1; SMUG1 and the HOX C 8–13 genes. (B) The human genomic sequence corresponding to the Sp7 cDNA is located on clone RP11-147A18 (accession AC021103) and has three exons. The ORF is indicated by closed boxes. The sequences around the initiation methionine codons (coloured blue) are shown, with those nucleotides corresponding to the Kozak consensus sequence coloured red. The stop codon and the double polyadenylation signal sequence are also shown. The size of the exons and introns are indicated. (C) The promoter region preceding exon 1a contains an S8 homeobox gene and Runx2/Cbfa1 potential cis-regulatory sites that are conserved in the mouse and rat Sp7 promoters. The promoter region preceding exon 1b contains a potential NF-Y CCAAT box binding site. The potential start of transcription is labelled TC and that of translation, TL. Positions are labelled relative to the start of translation. The Runx2/Cbfa1 and NF-Y sites are on the negative strand, (-).