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Figure 9 | BMC Genomics

Figure 9

From: Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

Figure 9

Real-time PCR (Lightcyclerâ„¢) based evaluation of the incubation time-dependent degradation of the two transcripts from Fig. 7and S27a. aRNA from the different incubation time-points (2 h, 4 h, 8 h and 16 h) was converted to cDNA and equal amounts were subjected to a real-time PCR with gene-specific primers (n = 3). The presence of a single PCR product was confirmed by melting curve analysis (data not shown) and agarose gel electrophoresis (inset; n.T. no template control; M 100 bp DNA marker). Numbers in columns refer to x-fold decrease compared to 4 h, calculated by a method described elsewhere [23].

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