Figure 5
From: TGF-β induces the expression of SAP30L, a novel nuclear protein
Transfection of the different EGFP fusion constructs to IMR-90 fibroblasts. A) The wild-type SAP30L concentrates in small dense bodies in the nuclei. B) The nuclear localization of the fusion protein with only the NLS of SAP30L provides evidence for the functionality of the nuclear localization signal. C) Mutating the NLS disrupts the nuclear localization of the protein to some extent. D) Anti-PML-antibody staining (red) of wild-type SAP30L-transfected cells shows that the nuclear concentrates are other than PML bodies.