Table 4 Different approaches used for identification of amplified genes in (IMR-32) neuroblastoma cell lines
Methodology | Publication | MYCN | DDX1 | NAG | NSE1 | g10d12 | DNMT3A | TEM8 | g4d5 | MEIS1 | g2h10 |
|---|---|---|---|---|---|---|---|---|---|---|---|
cloning after MYC Southern blot hybridization | Schwab et al. (1983) [18] | X | |||||||||
subtractive cDNA cloning and Southern blot | Manohar et al. (1995) [39] | X | |||||||||
differential cDNA library screening | X | ||||||||||
2D separation of genomic restriction fragments | Wimmer et al. (1999) [15] | X | |||||||||
screening PAC/BAC libraries after microdissection | Jones et al. (2000) [14] | X | |||||||||
serendipitous cloning after multiprobe Southern blot | Spieker et al. (2001) [13] | X | |||||||||
CGH on cDNA microarrays | Beheshti et al. (2003) [17] | X | X | X | X | X | |||||
combined subtractive cDNA cloning and array CGH | this study | X | X | X | X | X | X | X | X | X |