Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons

Table 4 Different approaches used for identification of amplified genes in (IMR-32) neuroblastoma cell lines

Methodology	Publication	MYCN	DDX1	NAG	NSE1	g10d12	DNMT3A	TEM8	g4d5	MEIS1	g2h10
cloning after MYC Southern blot hybridization	Schwab et al. (1983) [18]	X
subtractive cDNA cloning and Southern blot	Manohar et al. (1995) [39]		X
differential cDNA library screening	Godbout and Squire (1993); Squire et al. (1995) [19, 40]		X
2D separation of genomic restriction fragments	Wimmer et al. (1999) [15]			X
screening PAC/BAC libraries after microdissection	Jones et al. (2000) [14]									X
serendipitous cloning after multiprobe Southern blot	Spieker et al. (2001) [13]									X
CGH on cDNA microarrays	Beheshti et al. (2003) [17]	X		X	X		X			X
combined subtractive cDNA cloning and array CGH	this study	X	X	X	X	X		X	X	X	X

ISSN: 1471-2164