Figure 3

Triple-target: data consistency between dyes Relative standard deviation (or CV) of 3 values (one per dye-label) for each gene on an array plotted against the mean, showing intensity dependent variation of signal between the three dye-labels. Reproducibility of gene values across dye-labels begins to brake down at a low signal intensity of about 500. However, the last panel provides information about the distribution of CV values for each array, and it is evident that the majority of all genes on an array fall below 0.05 even before applying any normalisation methods.