Table 1 Progressive screening of SSH libraries.
SSH library | B. anthracis 7700 vs. B. cereus 14579T | B. anthracis 7700 vs. B. thuringiensis 10792T |
|---|---|---|
Number SSH clones tested | 2400 | 2000 |
Comparative colony filters selection (detection by tester probe > detection by driver probe) | 224 | 189 |
Clone DNA blot analysis (selection of clones not detected by driver probe) | 96 | 59 |
Initial genomic blot selection (blot contained B. anthracis 7700, B. cereus 14579T, B. thuringiensis 10792T and B. thuringiensis 97-27 genomic DNA probed with SSH clone insert DNA) | 32 | 20 |
Survey genomic DNA blot selection of B. anthracis -specific clones (blot contained 4 strains of B. anthracis and 8 other strains of B. cereus and B. thuringiensis – see Figure 1 and Table 2) | 27 | 12 |