Figure 2
From: Universal Reference RNA as a standard for microarray experiments
Gene expression of human brain and testis cell line KIAA0923 protein. Total RNA isolated from brain and testis cell lines were reverse-transcribed to cDNA, labeled with Cy5 and co-hybridized with Cy3-labeled UHRR onto two 43,000-spot cDNA microarrays (Stanford University). Each microarray had five separate spots containing the probes for KIAA0923 protein gene: (A) Comparison of Cy5 absolute fluorescence intensities registered on five KIAA0923 spots on arrays co-hybridized with Cy5-labeled brain and testis cDNA, and Cy3-labeled UHRR; (B) Comparison of Cy3 absolute fluorescence intensities registered on five KIAA0923 spots on arrays co-hybridized with Cy5-labeled brain and testis cDNA, and Cy3-labeled UHRR; (C) Ratios of intensity values in red and green channels (Cy5/Cy3 ratio); (D) Log2 of the Cy5/Cy3 ratios.