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Figure 3 | BMC Genomics

Figure 3

From: Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

Figure 3

Heatmap representation of normalized hybridization signals For each carbohydrate tested, background-subtracted hybridization signals were normalized between slides to the intensity of the controls and then assigned a number 0–3 depending on the normalized intensity. Signal intensities of 0–4999 were assigned a number of 0 and were colored black in the heatmap; those 5000–19,999 were assigned a number of 1 and were colored light red; those 20,000–39,999 were assigned a number of 2 and were colored medium red; and any intensities above 40,000 were assigned a number of 3 and were colored bright red to assist in visualization of the hybridization signals. Signal intensities of the selected cDNAs coding for heat shock protein (HSP), unknown peptide, phosphoenolpyruvate carboxykinase (PEPCK), galactomannoprotein, endo-arabinosidase and exopolygalacturonase are shown on the right hand side of the diagram.

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